Fast pfu buffer
WebPhusion ® High-Fidelity PCR Master Mix with GC Buffer For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo … WebNative Plus 10× Pfu buffer 1 ml 2 × 1 ml 4 × 1 ml a Sufficient native Pfu DNA polymerase is provided for up to 40 100- l reactions. b Sufficient native Pfu DNA polymerase is …
Fast pfu buffer
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WebPhusion DNA Polymerase Concentration: We generally recommend using Phusion DNA Polymerase at a concentration of 20 units/ml (1.0 units/50 μl reaction). However, the optimal concentration of Phusion DNA Polymerase may vary from 10–40 units/ml (0.5–2 units/50 μl reaction) depending on amplicon length and difficulty. Webspecified. At Gene Link, using the standard PCR buffer with KCl, a final dNTP concentration of 0.2 mM, a MgCl. 2. concentration of 1.5 is used in most cases. If the DNA samples contain EDTA or other chelators, the MgCl. 2. concentration in the reaction mixture should be raised proportionally. Given below is an MgCl. 2. concentration
WebFaster CFU units have been designed for the pharmaceutical industry to control the problem of powder contamination and reduce operator's exposure to powder; namely, the units protect the powder from blowing … WebHigh-Fidelity PCR Master Mix with HF Buffer. For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific ® …
WebPhusion HF Buffer Pack (F-518L) includes: • 5X Phusion HF Buffer (1X buffer provides 1.5 mM MgCl 2) • 50 mM MgCl 2 • DMSO Store at -20°C. Supply Center Convenient, on-site access to the products you need. Learn more. Customers who viewed this item also viewed F530S 128.00 Add to cart F530L 516.00 500 units Add to cart WebJun 12, 2024 · The PCR components were as follows: 4 μL of 5 × Fast-Pfu buffer, 0.8 μL of 2.5 mmol/L deoxyribonucleotide triphosphates (dNTPs), 0.8 μL of each primer (5 μmol/L), 0.4 μL of Fast-Pfu polymerase, and 10 ng of template DNA. The PCR conditions were 3 minutes of denaturation at 95 °C, followed by 27 cycles of 30 seconds at 95 °C, 30 …
WebPfu mix buffer is a proprietary formulation optimized for robust performance in PCR. Individual Pfu DNA Polymerase and Taq DNA Polymerase are available. Features. …
WebNative Plus 10× Pfu buffer 1 ml 2 × 1 ml 4 × 1 ml a Sufficient native Pfu DNA polymerase is provided for up to 40 100-μl reactions. b Sufficient native Pfu DNA polymerase is provided for up to 200 100-μl reactions. c Sufficient native Pfu DNA polymerase is provided for up to 400 100-μl reactions. Cloned Pfu DNA Polymerase Quantity elearning wsaibWebPhysical Dimensions. Show Reviews. Advance PBU 27 propane buffer. This buffer features a 27 buffing pad and 12V battery and key start. This buffer features an 18 HP … elearning wrdsbWeb5x FastPfu FLY Buffer (with Mg2+) – GK221 $ 33.00 CAD Format Buy 1 x 1.2 ml 5x Fly Buffer for 33 $ Buy 5 x 1.2 ml 5x Fly Buffer for 120 $ Buy 25 x 1.2 ml 5x Fly Buffer for … elearning wsbWebEach batch of TransStart® FastPfu Fly DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA. Restrictions For … elearningwseiWebDec 21, 2024 · The PCR reaction mixture including 4 μL 5 × Fast Pfu buffer, 2 μL 2.5 mM dNTPs, 0.8 μL each primer (5 μM), 0.4 μL Fast Pfu polymerase, 10 ng of template DNA, and ddH 2 O to a final volume of 20 µL. PCR-amplification cycling conditions were as follows: 95 °C for 5 min followed by 27 cycles of denaturation at 95 °C for 30 s, annealing … elearning wsb dgWebTransStart® FastPfu DNA Polymerase is a fast, high fidelity and high processivity hot start DNA polymerase. Brand TransStart® Characteristics - Extension rate is about 2-4 … e learning wsbWebFor high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific ® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust … e learning wsb dg